FIG. 4.

Ad-ISF35 IDI induces cytokine and chemokine release from the tumor microenvironment and spleen. We evaluated the level of expression of IL-2, IL-6, IL-10, IL12, GMCSF, IFNγ, and MCP-1 (pg/ml), among others, in samples collected from tumor, spleen, and serum from A20-bearing mice that received the vehicle, Ad5, or Ad-ISF35 IDI. The level of expression was quantified using the Luminex platform as described in the Materials and Methods section. Statistical analysis was performed using t-test with Welch's correction. *p<0.05; **p<0.01.