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. 2014 Nov 8;16:470. doi: 10.1186/s13058-014-0470-2

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© Jung et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sex-determining region Y (SRY)-box binding protein-2 (Sox2) can upregulate target gene transcripts in reporter responsive (RR) cells only and not reporter unresponsive (RU) cells. (A) MCF7 RU and RR cells were transfected with 3 μg of pcDNA3-Flag-Empty vector (EV), or pcDNA3-Flag-Sox2 (Sox2) and harvested for mRNA after 72 hours. Quantitative (q)-PCR analyses were performed using primers designed against MCF7 RR chromatin immunoprecipitation promoter microarray chip analysis (ChIP-chip) targets. Accompanying Sox2 qPCR analysis and Flag western blot shows transfection efficiency. (B) Western blot showing Sox2 knockdown efficiency in MCF7 RU and RR cells after 72-hour 20-nM scrambled or Sox2 siRNA treatment. q-PCR mRNA transcript analysis of MCF7 RU and RR cells after 72-hour 20-nM scrambled or Sox2 siRNA knockdown examining RR ChIP-chip genes in MCF7 RU and RR. (C) q-PCR analysis of MCF7 RU and RR cell PROM1 (CD133) transcripts after 16-hour treatments with dimethyl sulfoxide vehicle control or 10, 20, 30, or 40 nM Triptolide.