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. Author manuscript; available in PMC: 2015 Jan 22.
Published in final edited form as: Dev Biol. 2002 Dec 15;252(2):170–187. doi: 10.1006/dbio.2002.0847

Fig. 8.

Fig. 8

Sympathetic neurons overexpressing DN SHP-2 are dependent on NGF for survival in vitro. SCG neurons were cultured in the presence of 100 ng/ml NGF for 72 h; NGF was then removed prior to culture for an additional 96 h. Phase-contrast microphotographs of identical fields were taken immediately before and 96 h after NGF withdrawal. (A) Control (wt) dissociated neurons exhibit a dense network of processes prior to NGF withdrawal. (B) This same field is devoid of neurons 96 h after NGF withdrawal. (C) SHP-2 DN neurons have normal, phase-bright cell bodies after 72 h (black arrowheads), but process outgrowth is sparse (compare with A). (D) The neurons have disappeared 96 h after NGF withdrawal. Scale bar, 40 μm.