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. 2015 Jan 22;10(1):e0115007. doi: 10.1371/journal.pone.0115007

Figure 2. The representative time trace from the tethered particle motion experiments investigating the histone chaperone-mediated nucleosome assembly process.

Figure 2

(A) 7.5 nM histone octamer only. (B) 7.5 nM histone octamer along with 15 nM Nap1. (C) 7.5 nM histone octamer along with 15 nM Asf1.(D) 7.5 nM histone octamer along with 37.5 nM PGA. The black line indicates the initiation of reaction. The hatched bar represents the expected BM amplitude for a 836-bp DNA molecule in response to the addition of histone to form nucleosomes. The assembly trace displays a four-step shortening in DNA length.