Figure 6.

Multiple-turnover processivity assays to investigate the ability of AAG to search single-stranded DNA. (A) Schematic for the processivity substrates containing terminal fluorescein labels (asterisk) and two εA lesions (see Figure 2 for sequences). (B) Initial rates of substrate disappearance were measured for ssDNA and dsDNA processivity substrates with 2 nM full-length AAG and the indicated DNA (200 nM). (C) Processivity values for ssDNA and dsDNA (reference) were calculated from the initial rates of formation of the products and intermediates as described in Materials and Methods. Values are means ± the standard deviation (n ≥ 4). The action of AAG is distributive on ssDNA under every condition tested.