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. 2014 Dec 5;54(2):268–278. doi: 10.1021/bi5011249

Figure 7.

Figure 7

Quantitative analysis of transthyretin (TTR) amyloid fibrils formed in vitro in the presence of diflunisal (Dif.) and clusterin (CLU). Concentrations of amyloid-bound Congo red in (A) L55P TTR and (B) monoTTR fibril synthesis reaction mixtures pretreated with Dif., CLU, Dif. and CLU, or BSA, after a 10 day incubation period at 37 °C and pH 5.0. Corresponding wt rTTR treatments showed no fibril formation as wt tetramer is stable at pH 5.0 (data not shown). Data were background subtracted with respect to amount of Congo red bound to negative control in each treatment. **p < 0.005, and ***p < 0.001.