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. 2014 Dec 15;54(2):612–621. doi: 10.1021/bi501360e

Figure 6.

Figure 6

Effect of multivalent arsenicals on the reductase activity of PDI. (A) Schematic of the assay. Porcine insulin (50 μM) in 50 mM phosphate buffer, pH 7.5, containing 1 mM EDTA) was mixed with either 100 μM TCEP (B) or 5 mM GSH (C) in the absence or presence of 10 μM of As(III) in BA-1, BA-2, and TA-1. Time zero corresponds to the addition of 1 μM PDI (see Materials and Methods). The onset of turbidity was observed at 600 nm and is expressed in bar graph form in panels D and E (for B and C, respectively; see Materials and Methods).