Table 1. Quantitative Profile of αSyn Purification from Human Braina.
| purification step | total protein (μg/mL) | αSyn (μg/mL) | αSyn yield (μg) |
|---|---|---|---|
| total brain | 20889 (± 3100) | 18 (± 18) | 1432 (± 1484) |
| Ultra Supe | 4637 (± 2478) | 12 (± 6) | 850 (± 440) |
| SEC Start | 7707 (± 8105) | 81 (±46) | 653 (± 364) |
| AEC Start | 337 (± 221) | 7 (± 3) | 191 (± 75) |
| TS6b Start | 111 (± 40) | 61 (± 42) | 131 (± 68) |
| final | 205 (± 158) | 192 (± 128) | 96 (± 64) |
a
The concentrations of total protein and αSyn were determined at each stage of purification when starting with 20 g wet weight of human cortex. The yield of αSyn was calculated by multiplying the αSyn concentration (determined by an αSyn ELISA) by the volume at each step of the purification. Values represent the mean ± standard deviation from 4 independent experiments.