Table 3. Steady-State Kinetic Parameters for HIV-1 Subtype B (PRWT) and PRG48T/L89Ma.
Michaelis–Menten constants | |||
---|---|---|---|
subtype mutant | Km (μM) | kcat (s–1) | kcat/Km (s–1/μM–1) |
B-PRWT | 17 ± 3.9 | 3.8 ± 0.08 | 0.2 ± 0.02 |
B-PRG48T/L89M | 14 ± 1.6 | 3.0 ± 0.72 | 0.2 ± 0.07 |
The data are the average of three independent experiments.