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ITC data for the titration of (A) DB2277-AAAGTTT, (B) DB2277-AAGTT, and (C) DB2275-AAAGTTT. Injections of 10 μL aliquots of 50 μM ligand into 10 μM hairpin duplex DNA at 25 °C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA buffer, pH 7.4. The ITC raw data, located in the top panel, is the power output per injection as a function of time. The bottom panel is the peak integration of the data that shows the heat produced per injection as a function of the hairpin/ligand molar ratio.
