Table 1. Biosensor-SPR Equilibrium Dissociation Constants (K_D, nM) of DB2277 and Analogues with Pure A·T and Mixed DNA Sequences^a.

^aThe listed binding affinities are an average of two independent experiments carried out with two different sensor chips and the values are reproducible within 10% experimental errors. The experiments were conducted in Tris-HCl buffer (50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4) at 25 °C.