Table 2. Kinetics and Steady-State Analysis of DB2277 with the AAAGTTT Sequence at Different Salt Concentrations in 50 mM Tris-HCl, 1 mM EDTA Buffer, pH 7.4 at 25 °C^a.

	KD (nM)
[NaCl] mM	kinetic fit	steady state	ΔGb° (kcal/mol)	ΔHb (kcal/mol)	TΔSb (kcal/mol)
50d	0.2 ± 0.4		–13.2d	–4.7 ± 0.2	8.5
100	0.3 ± 0.2	0.2 ± 0.1	–13.0b	–4.2 ± 0.3	8.8
200	2.1 ± 0.6	4.3 ± 0.8	–11.8b	–4.3 ± 0.1	7.5
400	8.8 ± 0.9	9.5 ± 2.0	–11.0c	–4.1 ± 0.2	6.9
600	12.8 ± 2.0	13.2 ± 1.0	–10.7c	–3.7 ± 0.4	7.0

^aKinetic analysis was performed by global fitting with a 1:1 binding model.

^bData obtained from kinetic fit values.

^cData obtained from steady-state fit values; ΔH_b was determined in ITC experiments; TΔS_b = −ΔG_b^° + ΔH_b.

^dResults are calculated from extrapolation of log K_A vs log [NaCl/mM] (Figure 3E).