Table 3. Kinetics and Thermodynamic Results for DB2277 with the AAAGTTT Sequence at Different Experimental Temperatures at 50 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA Buffer at pH 7.4a.
|
KD (nM) |
|||||
|---|---|---|---|---|---|
| T (°C) | kinetic fit | steady state | ΔGb° (kcal/mol) | ΔHb (kcal/mol) | TΔSb (kcal/mol) |
| 20 | 0.8 ± 0.4 | 0.5 ± 0.3 | –12.4b | –3.1 ± 0.2 | 9.3 |
| 25 | 2.1 ± 0.6 | 4.3 ± 0.8 | –11.8b | –4.3 ± 0.1 | 7.5 |
| 35 | 4.2 ± 1.3 | 6.1 ± 0.7 | –11.6c | –5.1 ± 0.1 | 6.5 |
| 45 | 5.0 ± 0.8 | 8.2 ± 2.0 | –11.8c | –7.5 ± 0.2 | 4.3 |
a
Kinetic analysis was performed by global fitting with a 1:1 binding model.
b
Data obtained from kinetic fit values.
c
Data obtained from steady-state fit values; ΔHb was determined in ITC experiments; TΔSb = −ΔGb° + ΔHb