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NanoESI mass spectra of (a) acid-denatured apo-Mb (pH = 2.9), acid-denatured apo-Mb mixed with a 100 mM aqueous ammonium acetate solution at ratios of (b) 10:1 (pH = 3.9), (c) 2:1 (pH = 4.4), and (d) 1:1 (pH = 4.7), and (e) acid-denatured apo-Mb mixed with this ammonium acetate solution using a theta-glass emitter (estimated pH = 4.7). Percentages are the relative abundances of the folded fractions (7–9+ charge states) of apo-Mb.
