Figure 2.

Differentiation into EBs yields heterogeneous HORMAD1 and ZG16 expression levels. (A): Representative human embryonic stem cell (hESC)- and human induced pluripotent stem cell (hiPSC)-derived EBs at three different sizes using a standardized EB formation microwell plate (supplemental online Fig. 1B). Scale bars = 200 µm. (B): EBs made at different sizes (100, 1,000, and 10,000 cells per EB) were plated onto gelatin-coated plates and harvested at different points (1, 2, or 3 weeks after plating) for total RNA. Quantitative polymerase chain reaction (PCR) was performed. Overall low and heterogeneous expression of HORMAD1 was observed with no significant trend seen between the size or time samples (top). ZG16 expression was higher, and some lines at specific points had significant increases or decreases, although no significant trend was observed across the size or measurement points (bottom). Error bars represent ±SD. All PCRs were run in triplicate. Abbreviations: EB, embryoid body; ES, human embryonic stem cell line; iPS, human induced pluripotent stem cell lines; Undif. PSCs, undifferentiated pluripotent stem cells.