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. 2015 Jan 15;4(2):165–177. doi: 10.5966/sctm.2014-0179

Figure 1.

Figure 1.

Characterization of human embryonic stem cells (hESCs) cultured on Synthemax II-SC, Synthemax-R, and Matrigel. (A): Representative phase-contrast micrographs of H9 and H14 hESC colony morphology after nine passages on the indicated substrate. Scale bars = 200 μm. (B): Representative flow cytometry histograms for pluripotency markers Oct4 and SSEA4 in H9 cultures grown on the indicated substrate at passages 1, 3, 5, and 9. (C): Relative expression of pluripotency genes in H9 cultures grown on Synthemax II-SC, Synthemax-R, and Matrigel at passages 1, 3, 5, and 9 as detected by quantitative polymerase chain reaction. (D): Normal karyotype of H9 hESCs maintained on Synthemax II-SC for 23 passages. (E): Relative gene expression of germ layer markers is significantly higher in H9 hESCs differentiated on Synthemax II-SC for 10 days compared with hESCs at earlier passages. ∗∗, p < 0.01, t test. (F): H9 hESCs on Synthemax II-SC stained for nuclear pluripotency transcription factors Oct4 and Sall4 and surface markers Tra-1-81 and Tra-1-60. Scale bars = 80 μm. Abbreviation: Diff, differentiation.