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. 2015 Jan 15;4(2):165–177. doi: 10.5966/sctm.2014-0179

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Figure 2. — Spontaneous differentiation of retinal pigmented epithelia generated from human embryonic stem cells (hESC-RPE) on Matrigel and Synthemax II-SC yields significantly more pigmented area than hESC-RPE on Synthemax-R. Top: Pigmented area in individual wells was calculated with ImageJ software after 115 days of differentiation. Error bars denote standard deviation. ∗∗, p < .01, t test. Bottom: Three representative wells from a six-well plate of differentiated hESC-RPE seeded on each substrate are shown. Scale bar = 1 cm.