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. 2015 Jan 15;4(2):165–177. doi: 10.5966/sctm.2014-0179

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Figure 4. — Functional characterization of H9 RPE generated from human embryonic stem cells (hESC-RPE) derived on Synthemax II-SC. (A): Phagocytosis of ROS by H9 hESC-RPE derived on Matrigel, Synthemax-R, Synthemax II-SC, or Mg-SR. A function-blocking antibody against αvβ5 integrin, which is necessary for phagocytosis by RPE, significantly decreased internalization of ROS in all hESC-RPE when compared with the IgG isotype control. ∗∗, p < .01, t test. fRPE served as a positive control. (B): Phagocytosis of ROS by H9 hESC-RPE derived on Synthemax II-SC after seeding on the defined substrates Synthemax II-SC and human vitronectin. Phagocytic activity was significantly decreased with the function-blocking αvβ5 antibody when compared with the IgG isotype control. ∗∗, p < .01, t test. (C): All conditions secrete PEDF significantly more on the apical surface compared with the basal side when seeded on surfaces coated with human vitronectin or Synthemax II-SC. ∗∗, p < .01, t test. hESC-RPE derived on Synthemax II-SC secrete less apical PEDF compared with fRPE and cells derived on Synthemax-R. Abbreviations: fRPE, fetal retinal pigmented epithelium; HuVn, human vitronectin; ROS, rod outer segment; S2, hESC-RPE derived on Synthemax II-SC; Mg-SR, hESCs grown and differentiated on Matrigel and then enriched as hESC-RPE on Synthemax-R; PEDF, pigment epithelium-derived factor; SR, hESC-RPE derived on Synthemax-R.