Figure 2.

Flow cytometry analyses for SSEA-3 expression before and after enrichment of Muse cells using magnetic-activated cell sorting (MACS). An example of flow cytometry analysis performed to measure SSEA-3⁺ cells before and after MACS cell enrichment and separation is shown. Cultured human ASCs were processed using MACS separation to obtain SSEA-3⁺ cells. The positive and negative cell fractions after MACS separation were used as Muse-rich and Muse-poor cell populations, respectively, in subsequent experiments. Abbreviations: ASCs, adipose tissue-derived stem/stromal cells; SSEA-3, stage-specific embryonic antigen-3.