Figure 2. High affinity NP-specific antibodies decay more rapidly in the sera of CDH17^-/- mice.

CDH17^-/- mice and their WT littermates were immunized with NP-CGG in alum. (A, C) The titers of serum IgM (A) and IgG1 (C) antibodies against NP-BSA were measured in an ELISA. Antibody titers are expressed in terms of units derived from a panel of reference monoclonal NP-specific antibodies. Each symbol represents an individual mouse. The mean values are represented by the bold lines. The titers of high affinity (NP1.6), medium affinity (NP4), and total affinity (NP16) antibodies are shown. (B, D) Affinity ratios of IgM (B) or IgG1 (D) were calculated for high affinity (NP_1.6/NP₁₆) and medium affinity (NP₄/NP₁₆) antibodies using the data in (A) and (C). Statistical significance was tested using Student’s t-test (n = 15 (Week 4); n = 23 (other times); *P≤0.05, **P≤0.01). †Lower than 1 unit.