Table 2.
EC50 values for wild-type and mutant LasR activation by OdDHL and ligand 1
| mutation | OdDHL EC50 (nM) [a] | Ligand 1 EC50 (nM) |
|---|---|---|
| wild-type | 10 | 5 |
| T75V | 0.5 | 0.5 |
| Y93F | 10 | 10 |
| Y56F | 10 | 5 |
| S129A | 50 | 30 |
| W60F | 75 | 70 |
| R61M | 600 | 80 |
| T115V | 3500 | 3500 |
[a]
EC50 value is the concentration of OdDHL at which the activity is half the maximum activity for that mutant. Values are geometric means of biological triplicates (s.e.m. of log-transformed data ≤ ± 0.14, which corresponds to antilog errors of ×/÷ 1.4, n = 3). EC50 values that are > 3.6-fold different from each other (corresponding to log-transformed EC50 values that differ by > 0.56) are statistically significant (p < 0.05). Representative dose curves are shown in Figure S2.