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Biochemical fractionation reveals a pre-miRNA-degrading enzyme that competes with pre-miRNA processing.
(A) In vitro miRNA-generating assays performed with HeLa extract or soluble and pellet fractions following ammonium sulfate precipitation.
(B) Immunoblotting of fractions for Dicer and TRBP.
(C) Full-length, overexposed autoradiogram depicting pre-miRNA-degradation products.
