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. Author manuscript; available in PMC: 2015 Nov 20.
Published in final edited form as: Cell Rep. 2014 Nov 20;9(4):1471–1481. doi: 10.1016/j.celrep.2014.10.021

Figure 4.

Figure 4

Pre-miRNA degradation by TN/TX competes with pre-miRNA processing by Dicer/TRBP in vivo.

(A) Immunoblotting performed with testis extracts from two pairs of wild type (wt) and translin (tn) −/− mice.

(B) Pre-miRNA processing assays performed with 25 μg of testis extract from two pairs of wt and tn−/− mice.

(C) Northern blotting performed with cerebellar RNA from two pairs of wt and tn−/− mice. Blots were hybridized with probes for miR-409 and 5s rRNA.

(D) Quantitative analysis of primary (pri-), precursor (pre-) and mature miR-409 from wt (black bars) and tn−/− mice (gray bars). * indicates greater than wt (p ≤ 0.02; n = 3; means ± SD). See also Table S3.