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. Author manuscript; available in PMC: 2015 Jan 23.
Published in final edited form as: Cancer Res. 2010 Apr 13;70(8):3140–3149. doi: 10.1158/0008-5472.CAN-09-4456

Figure 4.

Figure 4

BBC3/PUMA is target of miR-483-3p. A, putative binding site of miR-483-3p in PUMA 3′UTRs (miRBase Target Database). Asterisks, nucleotides substituted in miR-483-3p predicted target site to perform luciferase assay (GGAGT>AATTA). B, PUMA 3′UTRs regulate luciferase activity dependent on miR-483-3p in HEK293, HCT116, and HepG2 cell lines (WT, wild-type; MUT, mutant; P, P value). Firefly luciferase activity was normalized on Renilla luciferase activity of the cotransfected pRL-TK vector. C, Western blot analysis of PUMA after miR-483-3p transfection in HEK293 and HepG2 cell line; the α (23 kDa) and β (18 kDa) isoforms are indicated. Cells were collected after 48 h of miRNA transfection. D, Western blot analysis of PUMA in Wilms’ tumor samples (T) and matched normal tissues (N).