Skip to main content
. Author manuscript; available in PMC: 2015 Jan 23.
Published in final edited form as: Cancer Res. 2010 Apr 13;70(8):3140–3149. doi: 10.1158/0008-5472.CAN-09-4456

Figure 5.

Figure 5

Stable cell lines overexpressing anti–miR-483-3p are more sensitive to apoptosis stimuli. HCT116 cells overexpressing the miR-483-3p (LV-483) or the anti–miR-483-3p (LV-AS483) and HepG2 cells overexpressing the anti–miR-483-3p were treated with different concentrations of 5FU. LV-CTRL indicates stable cell line with the empty vector. A, Western blot analysis reveals an increment of PUMA protein levels only in the LV-AS483 cells. B, caspase 3/7 activity was decreased in LV-483 cells and increased in LV-AS483 when compared with LV-CTRL cells. Data were normalized on LV-CTRL cells. C, relative caspase 3/7 activity in HepG2-LV-CTRL and LV-AS483 after treatment with 5FU and siPUMA (+) or siRNA scramble (−). Data were normalized on the average caspase activity of HepG2-LV-CTRL cells.