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. Author manuscript; available in PMC: 2015 Feb 1.

Published in final edited form as: Mol Cell Biochem. 2014 Oct 30;400(0):9–15. doi: 10.1007/s11010-014-2256-3

Fig. 3 — The position of the SvAS protein is indicated with a solid square. Albumin was run twice for this experiment. Inset (bottom panel), the results were obtained by removing an aliquot of the active fraction corresponding to a molecular mass of 67 kDa from the gel filtration column, adding it to the assay cuvette, and immediately measuring the change in fluorescence intensity (in counts per second) over time (in sec). The fact that no lag is observed at the beginning of the assay suggests that assembly of the monomers into higher-order structures (dimers) is not required for activity. See Materials and Methods for details

Fig. 3 — The position of the SvAS protein is indicated with a solid square. Albumin was run twice for this experiment. Inset (bottom panel), the results were obtained by removing an aliquot of the active fraction corresponding to a molecular mass of 67 kDa from the gel filtration column, adding it to the assay cuvette, and immediately measuring the change in fluorescence intensity (in counts per second) over time (in sec). The fact that no lag is observed at the beginning of the assay suggests that assembly of the monomers into higher-order structures (dimers) is not required for activity. See Materials and Methods for details