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. Author manuscript; available in PMC: 2015 Jan 23.
Published in final edited form as: J Invest Dermatol. 2014 Jan 31;134(7):1903–1911. doi: 10.1038/jid.2014.61

Figure 2. Allergen-specific IL-9 production by PBMC from nickel-allergic patients.

Figure 2

PBMC were isolated from 7 different patients with ACD to nickel (Table 1), and cultured with nickel chloride (1–10 μg/ml of culture medium) for ninety six hours. Supernatants were harvested, and the following cytokines were assayed in cell-free culture supernatants: IL-9, IFN-γ, IL-4, and IL-2 (data depicted represent mean +/− SD of samples assayed in triplicate). (A) Dose response curve to Nickel titration from two nickel allergic patients and two nickel tolerant patients. (B) IL-9 production from the same two nickel allergic and tolerant patients, demonstrating that both groups can produce IL-9 in an equivalent manner after PHA stimulation. (C) Scatter plot represents the peak cytokine production from the seven allergic patients. The scatter plot of peak cytokine production (horizontal bar represents mean value) for both IFN-γ and IL-9 were significantly higher than of IL-4 (P<0.001), with the average peak IL-9 production is similar to IFN-γ (P>0.05).