. 2014 Jul 15;9(2):273–285. doi: 10.1038/ismej.2014.119

Table 1. Primers and probes designed in this study for quantitative PCR and reverse-transcription quantitative PCR where R² is the correlation coefficient of the standard curve and E is PCR efficiency.

Target	Forward primer (5′-3′)	Reverse primer (5′-3′)	Probe (5′-3′)	R²	E (%)
OTU0001 (Pseudomonas)	CAAGGCCCAGAACACCATCA	TTCGAGGAAGTTGATCGCGG	AAATGGCGGCGGAGGCCG	0.932–0.989	97–104
OTU0002 (UCYN-A)	AGCTGCAGAAAGAGGAACTGT	GGCTCAGGACCACCAGATTC	TCCTGGTTACAACAACGTTTTATGTG	0.968–0.971	90–93
OTU0005 (Anabaena)	TCCAAGGCTCAAACAACCGT	CAACCTACACCGGGTTCTGG	CCGCGACGTTAAATGCGTGGAATC	0.926–0.935	95–106
OTU0009 (Klebsiella)	ACCATTATGGAGATGGCCGC	GCCAATTTGCAGCACGTCTT	TCGGCTCGGTCGAGGACCTCGA	0.971–0.972	92–100
OTU0014 (Bradyrhizobium)	GATGCTTGGTGGATTGGCAC	ACAGAGTTCCGCTAAAGCCC	TGCGAGAAGAAGGCGAGGACGTCGAG	0.949–0.995	92–93

Table 1. Primers and probes designed in this study for quantitative PCR and reverse-transcription quantitative PCR where R2 is the correlation coefficient of the standard curve and E is PCR efficiency.