Figure 1.

Neighbor-joining tree based on multilocus sequence analysis (MLSA) of genome-sequenced organisms of the Roseobacter clade. The tree was constructed using ARB v5.1 and is based on a similar tree of Newton et al. (2010) but includes additional genome sequences and an extended gene set. Filled circles indicate nodes also recovered reproducibly with maximum-likelihood calculation. Numbers at the nodes are bootstrap values (only >50% are shown) from 1000 replicates. Subclades of the Roseobacter clade are marked by different colors. (T) indicates type strains. Escherichia coli K12 MG1655 was used as outgroup.