Fig. 7.

Limited proteolysis of the soluble VSGs using S. aureus V8 protease. Samples of each purified protein (40 μg) were either left untreated (a), or partially digested with 1.12 (b) or 0.37 (c) units of S. aureus V8 protease for 4.5 h at room temperature. SDS-PAGE analysis of these samples was performed using a 15% (w/v) polyacrylamide gel. Gels were either silver stained (top panel) or analyzed by Western blotting using polyclonal antibodies produced in rabbit against the soluble VSG form from TEVA1 (middle panel), or serum from a bovine naturally infected with T. vivax (bottom panel).