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. 2014 Nov 25;290(4):1927–1935. doi: 10.1074/jbc.M114.608703

FIGURE 1.

FIGURE 1.

SUMO conjugates human β-arrestin 2 on Lys-295. A, the alignment of bovine/human/murine β-arrestin 2 sequences sowing the SUMO consensus motif. B and C, human β-arrestin 2 is SUMOylated in vivo. HEK293T cells were transfected with the indicated plasmids, and the transfected cells were harvested 36 h after transfection and immunoprecipitated with anti-FLAG M2-agarose beads or anti-HA beads. The immunoprecipitates (IP) and the original whole-cell lysates (WCL) were analyzed by immunoblotting (IB) with anti-HA or anti-FLAG antibodies. D, HEK293T cells lysates were immunoprecipitated with anti-β-arrestin 2 antibody or control IgG. The immunoprecipitates and the whole-cell lysates were analyzed by immunoblotting with anti-SUMO1 or anti-β-arrestin 2 antibodies. E, Lys-295 is the major SUMOylation site of human β-arrestin 2. HEK293T cells were transfected with the indicated plasmids, and the transfected cell lysates were immunoprecipitated with anti-FLAG M2-agarose beads, followed by Western blot analysis by using anti-ΗΑ or anti-FLAG antibodies.