FIGURE 3.

The kinase activity of AS PKCδ is specifically inhibited by 1NA-PP1. A, structure of PP1 analogs. B, WT or AS PKCδ was incubated with [γ-³²P]ATP, histone 3, and a 1 μm concentration of staurosporine (Stau), bisindolylmaleimide I (Bis), or PP1 analogs. Top, autoradiogram showing phosphorylated histone 3; bottom, histone 3 in a silver-stained gel. Inhibition of WT and AS PKCδ by staurosporine and bisindolylmaleimide I (C) and by PP1 analogs (D) was measured by fluorescence polarization assays (n = 3). Error bars, S.E.