FIGURE 6.
BI2536 and metformin synergistically inhibit LuCaP35CR xenografts. LuCaP35CR tumors were inoculated into nude mice that had been castrated 2 weeks prior. After tumors reached sizes of 250–300 mm3, mice were intravenously injected twice per week with BI2536 (BI; 30 mg/kg), given metformin (Met; 15 mg/kg) by gavage, or both and followed for an additional 43 days. A, images of the mice at the end of the study. B, tumor growth curves of the study. CTRL, control. C, serum PSA levels of the mice. D, tumor weight measurement upon sacrifice. E, immunoblot of the harvested tumor samples. c-PARP, cleaved PARP. *, p < 0.05; **, p < 0.01.