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Restoration of the inhibitory effect of mPIRO-1 on the formation of MNCs by exogenous PGRN. MBMMs were transfected with si-mPIRO2 and were further differentiated in the presence of RANKL with or without recombinant mouse PGRN. TRAP+ MNCs were counted under a light microscope (100×). *, p < 0.05, which was considered significant.
