FIGURE 2.

Theoretical search for a putative PI(4,5)P₂ binding site in the TRPV1 channel. A and B, top view and side view (with respect to the plane of the membrane) of the TRPV1 channel bound to four PI(4,5)P₂ molecules. C, atomistic representation of the PI(4,5)P₂ model. Numbers 1, 4, and 5 indicate the position of the phosphate groups. D, conformation of PI(4,5)P₂ in the binding pocket of the wild-type TRPV1 channel. Inset (dotted green lines), magnified view of the atomic-level interactions between wild-type TRPV1 and PI(4,5)P₂. Portions of two different subunits are shown. Amino acid residues Arg-575 and Arg-579 are located in the S4-S5 linker of the blue subunit and residue Lys-694 is in the TRP domain of the yellow subunit.