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Forskolin treatment leads to loss of ArgBP2 from stress fibers. A and B, a micrograph showing control cells treated with DMSO (A) and the effect of forskolin (B; 25 μm) on HeLa cells. Anti-ArgBP2, anti-α-actinin and phalloidin staining are shown; a loss of ArgBP2 from stress fibers is observed but the protein remains at focal adhesions. The fluorescent signals were inverted for greater clarity. The box marks the enlarged panel where stress fibers are clearly visible.
