TABLE 1.
EOGT construct | Forward primer (5′→3′) | Reverse primer (5′→3′) |
---|---|---|
EOGT W207S | GTTTGCTGAGCTACAAGGCT | GAAGACTGCAGGGGACTTTT |
EOGT R377Q | CAGAAAATCCTGAACCAAGAC | GTATTCTGTGCTGCGTGCAAG |
EOGT Δ359–527 | CATGATGAGCTATAGACTCGA | TCCCTCTTGAGTGATGTTCAG |
EOGTΔ359–527 + HDEL | TAGACTCGAGGAGGGCCCGAA | TCCCTCTTGAGTGATGTTCAG |