TABLE 2.

Summary of K_d values used in kinetic modeling with trimeric αSNAP₃

Reaction	Name	Kd	Source
αSNAP3 + SC ⇌ αSNAP3·SC	K20	100 nm	Direct measurement (Fig. 3C)
αSNAP3 + αSNAP3·SC ⇌ (αSNAP3)2·SC	K21	100 nm	Direct measurement (Fig. 3C)
αSNAP3 + (αSNAP3)2·SC ⇌ (αSNAP3)3·SC	K22	100 nm	Direct measurement (Fig. 3C)
αSNAP3 + (αSNAP3)3·SC ⇌ (αSNAP3)4·SC	K23	100 nm	Direct measurement (Fig. 3C)
αSNAP3·SC + NSF ⇌ αSNAP3·SC·NSF	K24	84 nm	Km of αSNAP3 for SC disassembly by NSF (Fig. 3D)
(αSNAP3)2·SC + NSF ⇌ (αSNAP3)2·SC·NSF	K25	84 nm	Km of αSNAP3 for SC disassembly by NSF (Fig. 3D)
(αSNAP3)3·SC + NSF ⇌ (αSNAP3)3·SC·NSF	K26	84 nm	Km of αSNAP3 for SC disassembly by NSF (Fig. 3D)
(αSNAP3)4·SC + NSF ⇌ (αSNAP3)4·SC·NSF	K27	84 nm	Km of αSNAP3 for SC disassembly by NSF (Fig. 3D)
αSNAP3 + NSF ⇌ αSNAP3·NSF	K28	390 nm	Km of αSNAP3 for the ATPase activity of NSF (Fig. 3E)
αSNAP3 + αSNAP3·NSF ⇌ (αSNAP3)2·NSF	K29	390 nm	Km of αSNAP3 for the ATPase activity of NSF (Fig. 3E)
αSNAP3 + (αSNAP3)2·NSF ⇌ (αSNAP3)3·NSF	K30	390 nm	Km of αSNAP3 for the ATPase activity of NSF (Fig. 3E)
αSNAP3 + (αSNAP3)3·NSF ⇌ (αSNAP3)4·NSF	K31	390 nm	Km of αSNAP3 for the ATPase activity of NSF (Fig. 3E)
αSNAP3·NSF + SC ⇌ αSNAP3·SC·NSF	K32	22 nm	Thermodynamic cycle ((K20 × K24)/K28)
(αSNAP3)2·NSF + SC ⇌ (αSNAP3)2·SC·NSF	K33	5.6 nm	Thermodynamic cycle ((K32 × K36)/K29)
(αSNAP3)3·NSF + SC ⇌ (αSNAP3)3·SC·NSF	K34	1.4 nm	Thermodynamic cycle ((K33 × K37)/K30)
(αSNAP3)4·NSF + SC ⇌ (αSNAP3)4·SC·NSF	K35	0.36 nm	Thermodynamic cycle ((K34 × K38)/K31)
αSNAP3·SC·NSF + αSNAP3 ⇌ (αSNAP3)2·SC·NSF	K36	100 nm	Thermodynamic cycle ((K21 × K24)/K25)
(αSNAP3)2·SC·NSF + αSNAP3 ⇌ (αSNAP3)3·SC·NSF	K37	100 nm	Thermodynamic cycle ((K22 × K25)/K26)
(αSNAP33·SC·NSF + αSNAP3 ⇌ (αSNAP3)4·SC·NSF	K38	100 nm	Thermodynamic cycle ((K23 × K26)/K27)