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αSNAP and αSNAP3 in steady-state NSF-mediated SC disassembly
SC disassembly was monitored in steady-state conditions with varying concentrations of αSNAP or αSNAP3 to determine their contribution to disassembly ([NSF] = 2 nm, [ATP] = 400 μm, [SC] = 50 nM, [αSNAP] = 0–2500 nm, [αSNAP3] = 0–590 nm).
| αSNAP + SC | αSNAP3 + SC | |
|---|---|---|
| kcatdisassembly (min−1) | 1.9 ± 0.14 | 1.6 ± 0.04 |
| KmαSNAP3 (μm) | 1.2 ± 0.19 | 0.084 ± 0.010 |
| kcatdisassembly/KmαSNAP3 (μm−1 min−1) | 1.7 ± 0.30 | 19 ± 2.3 |
