TABLE 5.

Pre-steady-state measurement of SC disassembly and ATP hydrolysis by NSF

These rate constants of ATP hydrolysis and SC disassembly were used to determine the number of ATP hydrolyzed per SC ([NSF] = 2 μm, [αSNAP₃] = 100 nm, [ATP] = 500 μm). The fraction of αSNAP₃ bound to SC was determined using the protein concentrations in each experiment in conjunction with the quadratic equation for binding (see “Experimental Procedures”).

[SC]	kobsATP, +αSNAP3	kobsATP, −αSNAP3	kobsATP, +αSNAP3 − kobsATP, −αSNAP3	kobsSC	αSNAP3 bound to SC
nm	min−1	min−1	min−1	min−1	%
37.5	1.47 ± 0.06	0.68 ± 0.11	0.80 ± 0.13	0.015 ± 0.001	30
75	2.17 ± 0.17	0.63 ± 0.12	1.54 ± 0.21	0.039 ± 0.001	54
150	2.81 ± 0.10	0.69 ± 0.10	2.12 ± 0.14	0.089 ± 0.002	79
300	3.40 ± 0.05	0.64 ± 0.10	2.76 ± 0.11	0.174 ± 0.006	92
450	3.44 ± 0.09	0.65 ± 0.13	2.79 ± 0.16	0.215 ± 0.012	95
600	3.86 ± 0.27	0.62 ± 0.12	3.24 ± 0.30	0.243 ± 0.017	97
750	3.94 ± 0.40	0.70 ± 0.11	3.24 ± 0.41	0.305 ± 0.019	97
900	3.53 ± 0.28	0.60 ± 0.15	2.93 ± 0.32	0.299 ± 0.026	98