FIGURE 3.

Hhat topology mapping using HA insert constructs. A, COS-7 cells were transfected with Hhat cDNA containing the indicated internal HA epitope tags. Cells were treated and visualized as in Fig. 2. B, COS-7 cells were transfected with Hhat cDNA with the indicated internal FLAG or HA epitope tags. Cells were treated as in Fig. 2 with one exception: staining of endogenous caveolin served as an internal control for a cytosolic epitope. Images for each construct were collected using the same conditions on the same day ensuring fair side-by-side comparison.