FIGURE 3.

Transcriptional repression of EZH2 by WT1. A, three putative WT1 recognition motifs were identified in the 1143-bp human EZH2 5′-flanking sequence (empty boxes, upper right corner). Different sets of primers were used to quantify the promoter or intron 1 of EZH2 after ChIP using a WT1-specific antibody. Quantification by PCR of immunoprecipitated DNA is shown on the left. TSS, transcription start site. B, amMSC stably expressing WT1B isoforms were transiently transfected with the full-length (−1095 to +48) and the two truncated EZH2 promoter/luciferase constructs; normalized reporter luciferase activity is expressed as the percentage of empty vector control. ANOVA with post hoc t test, p < 0.001(**), not significant (NS).