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. 2014 Oct 20;290(4):2279–2288. doi: 10.1074/jbc.M114.573576

FIGURE 4.

FIGURE 4.

Effect of WT1 or siEZH2 on β-catenin expression and canonical WNT9b response. A, CTNNB1 mRNA was measured by qRT-PCR in amMSC transfected with WT1B versus empty vector control (left panel) and in amMSC transfected with siEZH2 versus scrambled siRNA negative control (siNegCtrl, middle panel). A representative agarose gel of EZH2 and CTNNB1 qRT-PCR products is shown. EZH2 transcript level normalized to β2-microglobulin (B2M) is shown in the presence or absence of siEZH2 (right panel).B, amMSCWT1B were transiently transfected with the Super 8X TOPFlash reporter construct and exposed to L cells or L cells expressing WNT9b for 48 h. Luciferase activity was normalized to a co-transfected Renilla reporter. t test, p < 0.0001 (†), p < 0.001 (****), not significant (NS).