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. 2014 Oct 20;290(4):2279–2288. doi: 10.1074/jbc.M114.573576

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Effect of WT1B or siEZH2 on dynamic expression of genes involved in nephrogenesis. A, in amMSC transfected with WT1b or empty vector control, transcript levels for various markers validated by GUDMAP data were quantified by qRT-PCR normalized for β2-microglobulin (B2M): stem cell maintenance markers (POU5F1 and NANOG); uninduced progenitor cells of cap mesenchyme (SIX2, PAX2, and SALL1); mesenchyme-to-epithelium markers during early stages of nephrogenesis (RAR-α, PAX8, WNT4, FGF8, and LHX1); and terminal differentiation of the proximal tubule (SLC3A1 and AQP1). B, the same genes were quantified in amMSC transfected with siEZH2 versus siNegCtrl. t test, p < 0.0001 (†). NS, not significant.