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Analysis of the Arg102–Glu1032 salt bridge using surface plasmon resonance. A and B, wild type C3d (E1032) and mutant C3d (A1032) analytes were injected over amine-coupled immobilized C3c as ligand in 50 mm NaCl (red) and 137 mm NaCl (black) buffers. C, corresponding KD fits for 50 mm NaCl (red) and 137 mm NaCl (black). C3d (E1032) gave a KD value of 51 μm in 50 mm NaCl buffer.
