FIGURE 2.

Purification of the five proteins. A, the elution profiles in 137 mm NaCl buffer of C3 (continuous gray line), C3u (dashed gray line), C3b (continuous black line), and C3c (dashed black line) are shown at the top, and that for C3d is shown at the bottom. B, non-reduced and reduced SDS-PAGE analyses of the five proteins in 137 mm NaCl buffer. The α and β chains of C3, C3u, and C3b are labeled, together with the masses of the protein markers.