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RT-PCR of clp genes during reaeration. RT-PCR of clp proteases during reaeration clpP1 (a), clpP2 (b), clpC (c), and clpX (d) is shown. Induction of clpP1, clpP2, and clpC occurred by 1 h post-reaeration Mtb. However, there was no induction of clpP1, clpP2, and clpC throughout reaeration treatment within the isogenic mutant, Mtb:ΔRv2745c. clpX expression -fold change was <1 for all time points and strains throughout reaeration.
