TABLE 1.

Thermodynamic parameters of guanidine HCl induced unfolding of α-synuclein fibrils

All of the values in the table are means ± S.D. of three independent experiments. The values are as follows: [D]_50% (m), midpoint concentration of guanidine HCl; ΔG_el⁰ (kJ mol⁻¹), free energy of association in absence of denaturant; m (kJ mol⁻¹ m⁻¹), dependence of ΔG_el on denaturant concentration; c_c, critical concentration (μm).

α-Synuclein	[D]50%	ΔGel0	m	cc
	m	kJ mol−1	kJ mol−1 m−1	μm
WT	1.2 ± 0.1	−30.8 ± 0.1	5.6 ± 0.5	4.1 ± 0.1
H50Q	2.4 ± 0.1	−35.4 ± 0.9	4.5 ± 0.3	0.6 ± 0.2