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The BubR1 Phe and D boxes contribute to MCC homeostasis in human cells. Anti-Myc, anti-Bub3, anti-Cdc20, and anti-Mad2 blots of the lysates (Input) and anti-Myc immunoprecipitates (IP) from mitotic HeLa cells transfected with the indicated Myc-BubR1 plasmids and treated with nocodazole or taxol and MG132. The relative Cdc20 and Mad2 intensities of the IP samples were quantified and are indicated below each lane.
