FIGURE 3.

Effects of LDL receptor overexpression on the clearance of S1P and apoM in vivo. Clearance of C₁₇S1P bound to apoM-containing lipoproteins (A–E) and C₁₇S1P bound to apoM-depleted lipoproteins (F) in vivo. C₁₇S1P bound to apoM-containing lipoproteins or apoM-depleted lipoproteins was administered to mice infected with Ad-null or Ad-LDLr via the tail vein. The analyses were performed at 30 min after administration. A, plasma C₁₇S1P levels; B, the distribution of C₁₇S1P among FPLC fractions; C and D, plasma levels of exogenously administered human apoM and innate mouse apoM; and E, the distribution of exogenously administered human apoM among FPLC fractions, when mice were injected with C₁₇S1P bound to apoM-containing lipoproteins (n = 4/group). F, plasma C₁₇S1P levels when mice were injected with C₁₇S1P bound to apoM-depleted lipoproteins (n = 5–6/group).